Fig. 5: HrAgo1 mediates RNA silencing in human cells.
From: RNA-guided RNA silencing by an Asgard archaeal Argonaute
a A schematic diagram for stable transfection of miR-1-1. pLKO.1 puro-pri-mir-1-1 vector was transfected into AGO1/2/3 KO HCT116 cells, which were subsequently subjected to puromycin selection for 16 days to generate cells that stably express miR-1-1. b A schematic diagram for the RNAi rescue experiment. Puromycin-selected cells were co-transfected with a dual-luciferase expression vector containing two perfect target sites for miR-1-1 in the 3′ UTR of the firefly luciferase gene (Fluc) and a protein expression vector encoding sfGFP or hAgo2 or HrAgo1. Two days after the transfection, total RNA was isolated and subjected to RT-qPCR. c qPCR results for relative mRNA expression levels between firefly luciferase (Fluc) and Renilla luciferase (Rluc). Bars indicate mean ± SD (n = 3, biological replicates). ns, not significant; **p < 0.01; *p < 0.05 by independent two-sided t-test. The p values are: 0.725 (hAGO2 vs. HrAGO1), 0.014 (sfGFP vs. hAGO2), and 0.0064 (sfGFP vs. HrAGO1). Source data are provided in the Source data file.
