Fig. 7: Fully automated reconstruction of mitral/tufted cell axons at a millimetre scale. | Nature Communications

Fig. 7: Fully automated reconstruction of mitral/tufted cell axons at a millimetre scale.

From: Automated neuronal reconstruction with super-multicolour Tetbow labelling and threshold-based clustering of colour hues

Fig. 7

a A 3 × 5 tiled image (A z-stacked image of 2629.55 × 1636.36 × 437.31 μm3) of the lateral olfactory tract and olfactory cortex, with mitral and tufted cell axons labelled with 7-colour Tetbow (13-week-old male). Mitral/tufted cell-specific Pcdh21-Cre mice and FLEX-tTA AAV was used to label mitral/tufted cells with AAV-TRE-XFP. Images taken with a ×20 objective. 7 XFP images are merged after linear unmixing. Note that the data are from a representative result from four independent experiments with similar results. b Representative clusters of the three types after QDyeFinder at Th(d) = 0.25. Clusters were either clearly single neuron, containing few fragments, or containing axons from multiple neurons. See supplemental data for more details. c All 14 single neuron clusters (left panel) and their positions in UMAP space (right panel). Colours correspond to each cluster. See Supplementary Fig. 11 for individual plots.

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