Fig. 8: Reconstruction of fine axonal collaterals.

a Cartoon schematic, we injected 7-colour Tetbow AAVs into the olfactory bulb with the same methodology as Fig. 7. This time, we imaged the branches that innervate the posterior piriform cortex with a x63 objective to obtain a higher resolution. The mouse was 16-week-old male. b Individually adjusted maximum intensity projections of the x63 image for each of the XFPs used. c Summary of the percentages of clusters that were determined to include a single axon, a few fragments, or multiple complete axons. A Th(d) between 0.175 and 0.225 appears to be best. d Representative clusters of our QDyeTracer pipeline produced 28 clusters at a Th(d) of 0.2. Example clusters that display a single axonal tract (left panels), a few fragments (middle panels), and multiple axons (right panels). Cluster 18 does not represent axons (maybe autofluorescence). Note that multiple axons found for a cluster may in fact originate from the same neuron because we imaged a limited area in this case. e Illustration of all the single and multiple axon clusters (left) and in UMAP reduced colour space (right) Poorly defined clusters are labelled in grey. Note that most of the poor clusters contained very few fragments (categorized as “partial”; most likely autofluorescence or noise signals). Source data are provided as a Source Data file.