Fig. 6: Preventive NAC administration alleviates Icos-/- NOD mice myositis.
From: IFNγ causes mitochondrial dysfunction and oxidative stress in myositis
Icos-/- NOD mice were treated with NAC (2 g/L) from 14 weeks of age until 34 weeks of age (n = 10 mice/group). a Clinical score. b Percentage of disease-free mice. c Locomotor activity (Catwalk XT). d Grip strength. e Muscle strength after sciatic nerve stimulation. f Muscle weight. g Histopathological analysis. COX, NADH-TR, and SDH representative histoenzymology stainings (scale bars, 200 µm) and CD45/laminin immunofluorescence staining (scale bars, 1 mm). Graphs (right) depict the percentage of COXhigh fibers and the mean CD45-positively stained area with respect to total muscle area. Icos+/+ NOD (n = 5), Icos-/- NOD (n = 5), Icos-/- NOD + NAC (n = 7). h EPR measurement of ROS production (Icos+/+ NOD (n = 4), Icos-/- NOD (n = 10), Icos-/- NOD + NAC (n = 10)). i Chemokine and cytokine mRNA expressions (arb. units: arbitrary units). Icos+/+ NOD (n = 6), Icos-/- NOD (n = 6), Icos-/- NOD + NAC (n = 8). For a and c, statistical analysis was performed using Two-way ANOVA and Sidak’s multiple comparison post-hoc test. For b, Log-rank (Mantel Cox) test was used. For d, statistical analyses were performed using Two-way ANOVA with Sidak’s post-hoc test and for e–i, statistical analyses were performed using the Kruskal–Wallis test with uncorrected Dunn’s multiple comparison test. For all panels, n values correspond to the number of independent mice. Data correspond to the mean values ± s.e.m and numbers on panels denote p values. For all, a representative experiment out of two is shown. Source data are provided as a Source Data file.
