Fig. 3: Assessment of TadA-8e derived cytosine base editors and dual base editor in tomato cells.

a Editing efficiency of TadA-8e derived cytosine and dual base editors at four target sites in tomato. Dots represent individual values, and bars represent mean ± SD of three biological replicates. b C-to-T editing efficiency of select base editors in tomato protoplasts at four target sites. Each dot represents a biological replicate. The data for each column include three biological replicates of four tomato loci. Different letters indicate significant differences (P < 0.05; one-way ANOVA, Duncan test). The maxima, centre, and minima of box the refer to Upper quartile, median, and Lower quartile. The maxima and minima of whiskers refer to maximum value and minimum value. c A-to-G editing efficiency of select base editors in tomato protoplasts at four target sites. Each dot represents a biological replicate. The data for each column include three biological replicates of four tomato loci. Different letters indicate significant differences (P < 0.05; one-way ANOVA, Duncan test). The maxima, centre, and minima of box the refer to Upper quartile, median, and Lower quartile. The maxima and minima of whiskers refer to maximum value and minimum value. d Indels efficiency of select base editors in tomato protoplasts at four target sites. Each dot represents a biological replicate. The data for each column include three biological replicates of four tomato loci. Different letters indicate significant differences (P < 0.05; one-way ANOVA, Duncan test). The maxima, centre, and minima of box the refer to Upper quartile, median, and Lower quartile. The maxima and minima of whiskers refer to maximum value and minimum value. e Editing window of six base editors at site SIBlc-sgRNA01 and SIBlc-sgRNA02. The editing efficiencies of C-to-T and A-to-G base editing at 4 sites, ranging from positions 1–20, were fitted together to obtain the editing window as shown in the figure. Data are presented as mean values ± SEM. f Genotyping of protoplasts edited by TadDE at site SIBlc-sgRNA01 and SIBlc-sgRNA02. Lowercase red letters indicated base editing outcomes. The values on the right represent ratio and reads of mutation alleles. Source data are provided as a Source Data file.