Fig. 7: Engineering microRNA resistant OsSPL14 by TadDE-introduced synonymous mutations. | Nature Communications

Fig. 7: Engineering microRNA resistant OsSPL14 by TadDE-introduced synonymous mutations.

From: High performance TadA-8e derived cytosine and dual base editors with undetectable off-target effects in plants

Fig. 7

a Schematic of the target sites in OsSPL14. b Illustration of introducing OsSPL14 synonymous mutations to gain resistance to microRNA-mediated degradation without altering protein sequence. c Genotypes of engineered microRNA resistant OsSPL14 mutations in T0 lines. d Quantitative RT-PCR result of OsSPL14 transcripts in edited T0 rice lines. Each target contains three biological replicates. Data are presented as mean ± SD The statistical significance of differences was calculated using the one-way ANOVA analysis. Source data are provided as a Source Data file.

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