Fig. 1: Fgl2 expression in CD8⁺ T cells is tightly associated with exhaustion and portends increased mortality in human patients with melanoma.

a Representative flow plots and b summary data showing frequency of Fgl2⁺ cells within PD-1⁻ vs. PD-1⁺ CD8⁺ T cells from mice fourteen days post B16F10 melanoma challenge (p = 0.0006). Summary data showing frequency of c Ki-67⁺ (p = 0.0256), d TCF-1⁺ (p = 0.0006), and e Eomes⁺ (p = 0.0006) cells within Fgl2⁻ vs. Fgl2⁺ CD8⁺ T cells from B16-challenged mice. Representative data from two independent experiments, n = 7 mice per group. f Summary data and tSNE plot from a publicly available single cell RNA-sequencing dataset deposited by Carmona et al. visualizing Fgl2 gene expression of CD8⁺ tumor-infiltrating lymphocytes (TIL) isolated from B16F10 challenged mice (n = 7). t-SNE visualization and accompanying comparison of exhausted-like gene signature (Pdcd1 and Havcr2) with Fgl2 expression on g naive CD8⁺ T cells (n = 1249 cells), h PD-1^int effector CD8⁺ (n = 1548 cells), and i PD-1^high exhausted CD8⁺ T cells (n = 588 cells). For each comparison, p < 0.0001). j Schematic and summary data of Fgl2 expression on k macrophages (p < 0.0001), l regulatory T cells (Treg) (p = 0.6819), and m exhausted CD8⁺ T cells (p = 0.048) correlated to patient survival from a publicly available dataset deposited by Sade-Feldman et al. consisting of 16,291 single cell transcriptome profiles from patient tumors (n = 32 patients), data was normalized to housekeeping gene expression within each cell is shown. Mann-Whitney non-parametric, unpaired two-sided tests was used when comparing two groups, Kruskall-Wallis non-parametric, one-way ANOVA with Dunn’s multiple comparisons test was used when comparing >2 groups. The error bar in summary figures denotes mean ± SEM. *p < 0.05 ***p < 0.001, ****p < 0.0001. Source data are provided as a Source Data file. j was created with BioRender.com released under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International license.