Fig. 3: Dominant effect of TENT5B C-terminal GFP knock-in.

A Expression pattern of TENT5B-GFP, TENT5C-GFP, and GFP-TENT5B in GV oocytes visible as fluorescence of GFP. Scale bar = 50 µm. B Comparison of TENT5B and TENT5C expression levels depending on the presence of the GFP tag at the N- or C-terminus of proteins, measured as a GFP fluorescence intensity. Individual data points and n values represent individual oocytes analyzed, bars represent mean intensity values, error bars represent SEM; p values reported for comparison of mean values in the t-test, two-tailed. C Birth rate for different mating configurations depending on male and female C-terminal Tent5b^GFP genotype, presented as the number of pups born by individual females per week. Individual data points and n values represent individual females observed for 10 weeks, bars represent mean value, error bars represent SEM; p values reported for comparison of the number of pups in the Mann–Whitney–Wilcoxon test, two-sided. D, E Chromosome scatter preparations showing chromosome conditions and organization in Tent5b^wt/wt and Tent5b^wt/gfp MII oocytes. N values represent the number of individual oocytes analyzed; p value reported for comparison of the ratio of oocytes with one group of chromosomes to oocytes with chromosomes separated into two or more groups in Fischer’s exact test, two-tailed. F Chromatin (magenta) and alpha-tubulin (green) immunofluorescence staining in Tent5b^wt/gfp MII oocytes. Visible are examples of different chromosomal organization errors and other aberrations occurring after oocyte ovulation. Scale bar = 50 µm. G Frequency distribution of different chromosomal segregation and chromatin organization abnormalities in Tent5b^wt/gfp MII oocytes. N values represent individual oocytes analyzed; p value reported for comparison of the ratio of normal to abnormal oocytes in Fisher’s exact test, two-tailed. Source data are provided as a Source Data file.