Fig. 6: TENT5s enhance the expression of secreted proteins during gametogenesis.

A Differences in poly(A) tail profiles of transcripts containing CPEB1 motif in 3′UTR, compared to the rest of the transcriptome detected by DRS. In ovaries, analyses were performed for 522 oocyte-enriched mRNAs. Two-sided Mann–Whitney–Wilcoxon rank sum test was calculated. Dashed lines indicate the mean poly(A) tail lengths: Tent5b^−/− Tent5c^−/− = 115 nt; Tent5b^wt/wt = 111 nt; Tent5b^wt/gfp = 106 nt; Tent5b^gfp/gfp = 99 nt for transcripts with CPEB1, and Tent5b^−/− Tent5c^−/− = 106 nt; Tent5b^wt/wt = 104 nt; Tent5b^wt/gfp = 94 nt; Tent5b^gfp/gfp = 87 nt for transcripts without CPEB1 B Gene ontology (GO) analysis of potential TENT5 protein targets. C Distribution of poly(A) tail lengths in potential targets of TENT5 encoding proteins with and without signal peptide (SP). Dashed lines indicate the mean poly(A) tail lengths: Tent5b^−/− Tent5c^−/− = 65 nt; Tent5b^wt/wt = 94 nt; Tent5b^wt/gfp = 110 nt; Tent5b^gfp/gfp = 109 nt; Tent5c^−/− = 106 nt; Tent5c^+/+ = 168 nt; Tent5d^−/− = 92 nt; Tent5d^+/+ = 119 nt for substrates with SP, and Tent5b^−/− Tent5c^−/− = 63 nt; Tent5b^wt/wt = 85 nt; Tent5b^wt/gfp = 101 nt; Tent5b^gfp/gfp = 103 nt; Tent5c^−/− = 103 nt; Tent5c^+/+ = 115 nt; Tent5d^−/− = 100 nt; Tent5d^+/+ = 109 nt for substrates without SP. P values for TENT5B/C substrates with and without SP were calculated by two-sided Mann–Whitney–Wilcoxon test with Bonferroni Hallberg correction, and all p < 2.2e−12. P values for TENT5C and TENT5D substrates with and without SP were calculated by two-sided Mann–Whitney–Wilcoxon rank sum test, and all p < 2.2e−12 D Normalized mean intensity of fluorescence and calculated translation rates of oligoadenylated reporter YPET mRNAs with SP injected into Tent5b^+/+ Tent5c^+/+ and Tent5b^−/− Tent5c^+/− GV oocytes. Mean intensity data points represent mean YFP fluorescence intensity normalized to fluorescence of control polyadenylated reporter mRNA encoding mCherry. Individual translation rate data points and n values represent individual oocytes analyzed; bars represent mean values; error bars represent SEM; p values reported for the two-tailed t-test. Source data are provided as a Source Data file.