Table 1 Summary of fertility phenotypes discovered for each knock-out and knock-in mutation of Tent5 genes
Male | Female | |
|---|---|---|
Tent5a−/− | Lowered fertility due to poor overall animal condition | Infertility due to poor overall animal condition |
Tent5b−/− | Fertile | Fertile |
Tent5c−/− | Loss of sperm head and DNA material; Fewer total germ cells produced | Fertile |
Tent5d−/null or Tent5d−/− | Abnormalities in the development of primary spermatocytes; Degeneration of testes tissue; Lowered blood testosterone levels | Unknown—no homozygous females were analyzed due to Tent5d localization on chromosome X |
Tent5b−/− Tent5c−/− | Recapitulated Tent5c−/− phenotype | Inhibited ovarian follicle growth; No ovulation; Degeneration of preovulatory prophase I oocytes |
Tent5bgfp/gfp (N-terminal) | Fertile | Fertile |
Tent5bgfp/gfp (C-terminal) | Fertile | Abnormalities in spindle and chromatin organization in metaphase II oocytes; No ovulation |
