Fig. 2: Results of biological evaluations of hydrazone library. | Nature Communications

Fig. 2: Results of biological evaluations of hydrazone library.

From: Development of a natural product optimization strategy for inhibitors against MraY, a promising antibacterial target

Fig. 2

a General structures of hydrazine fragment moiety are shown. Each hydrazine class are represented by different colours (green; BZ, cyan; PA, grey; AC, yellow; AA, orange; LA). b–h MraY inhibitory activity and antibacterial activity of the hydrazone library. The bar graphs show the relative enzyme activity with DMSO as 100% when the compound is treated at given concentration (b,c; TUN at 100 nM, d,e; MRY at 10 nM, f.g; MRD at 100 nM, h; CAP at 1000 nM. n = 1), with the lower bars indicating higher inhibitory activity. The colour of the bars corresponds to Fig. 2a. Antibacterial activity was evaluated by the minimum inhibitory concentration (MIC) at three points, 0.5, 5, and 50 μM (n = 1), and shown as a heat map with the compound on the horizontal axis and the bacterium on the vertical axis. The redder the heat map, the lower the MIC value, i.e., the higher the antibacterial activity (pink; <0.5 µM, orange; 5 µM, yellow orange; 50 µM, light grey; >50 µM). Data of control compounds (van: vancomycin, col: colistin) are shown left column in each panel. E. faecium ATCC 35667, S. aureus ATCC 29213, P. aeruginosa ATCC 27853, K. pneumoniae ATCC 13883, E. cloacae ATCC 13047, A. baumannii ATCC 19606, E. coli ATCC 25922.

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