Fig. 4: H3R2C and H3R26C histone mutants disrupt transcriptional regulation of developmental and growth pathways. | Nature Communications

Fig. 4: H3R2C and H3R26C histone mutants disrupt transcriptional regulation of developmental and growth pathways.

From: Cancer-associated Histone H3 N-terminal arginine mutations disrupt PRC2 activity and impair differentiation

Fig. 4

A Gene Ontology analysis (TopGo) of differentially expressed genes when comparing H3R26C to H3WT or B genes differentially expressed in both H3R2C and H3R26C when each were compared individually to H3WT and when the direction of change was the same in both mutants. Red lettering indicates pathways relevant to linage specification or differentiation. C Change in gene expression at H3K27me3 associated genes based on H3K27me3 differential peak status (gain, loss, or no change; number of peaks denoted in parentheses) in H3R26C versus H3WT. p-values are shown above the brackets for the pairwise comparison between groups based on a two-sided Wilcoxon test. D Motif-analysis of sequences associated with H3K27me3 peak loss revealed putative MYOD1 and MYOG consensus sequences using the CIS-BP 2.00 database. E Gene set enrichment analysis of H3R26C vs H3WT demonstrated enrichment for genes in the myogenesis pathway from MSigDB Hallmarks (NES = 2.18, q-value = 3.9 ×10−9).

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