Fig. 3: Glycan binding analysis of Vic20 HA WT and mutants.
From: Epistasis mediates the evolution of the receptor binding mode in recent human H3N2 hemagglutinin
a Glycan array analysis of recombinant Vic20 HA proteins (WT and mutants, y-axis). Binding activity was measured by relative fluorescence unit (RFU) and represented by a heatmap. The corresponding glycan for each glycan ID (x-axis) is shown in Supplementary Data 1. b–f Binding avidities of b–e recombinant Vic20 HAs (WT and mutants) as well as f sambucus nigra agglutinin (SNA, positive control) to the indicated glycans were measured by ELISA. LN3-L was used as a negative control. The means of optical density 450 nm (OD450) from three independent experiments are shown with SD indicated by the error bars. -N: N-linked, -L: linear. Glycan diagrams are drawn according to the Symbol Nomenclature for Glycans recommended by the NLM61,62. Raw data are provided as a Source Data file.
