Fig. 2: Different homeostatic rebound after mechanical or social sleep deprivation.
72 h sleep profile (left) and quantification of rebound (right) in flies subjected to 24 h of mechanically-induced sleep deprivation (a), or 24 h of socially-induced sleep deprivation (b). Each panel features one strain from each of the seven wild-caught species, or CantonS. In all panels, the sleep profile of rested flies is shown as a continuous line, while sleep-deprived animals are shown in a dashed line. The sleep rebound at ZT 0–3 is quantified on the right side of each sleep profile. Numbers of animals (Ns) and p values of sleep-deprived vs. control are shown below each panel. Pairwise comparisons were conducted using the exact version of the Wilcoxon rank-sum test with false discovery rate (FDR) adjustment. The shaded areas indicate the bootstrapped 95% confidence intervals. c sleep stage analysis of all species during rebound time (ZT 0–3) after mechanical sleep deprivation. Increase in sleep depth is observed only in the laboratory strain of D. melanogaster. Ns are D. mellab: 200; D. melwild 78; D. sim 76; D. sec 72; D. ere 74; D. yak 69; D. wil 60; D. vir 72. The shaded boxes indicate the median and the interquartile range; the dots indicate the single data points and define minimum and maximum values. Quantification of the change in BRP expression in the brain of sleep-deprived flies after mechanical (d) or social male-male (e) sleep deprivation. Numbers of animals (Ns) and p values of sleep-deprived vs. control are shown below each panel in (d) and (e). ***p < 0.001; **p < 0.01; *p < 0.05. The shaded boxes indicate the median and the interquartile range, the whiskers define minimum and maximum values; the dots indicate the single data points. Pairwise comparisons were conducted using the exact version of the Wilcoxon rank-sum test with false discovery rate (FDR) adjustment.
