Fig. 2: BnaA07.MKK9 plays a positive role in the regulation of Sclerotinia stem rot (SSR) resistance in oilseed rape.
From: Natural variation in BnaA07.MKK9 confers resistance to Sclerotinia stem rot in oilseed rape
a–d Evaluation of SSR resistance in overexpression (OE) lines of BnaA07.MKK9 (T1 progeny) on seedling stage leaves (a, b) and mature stage stems (c, d). Disease lesions on leaves and stems were photographed and quantified at 48 and 168 h post-inoculation (hpi), respectively. Empty vector (EV) transgenic plants and wild-type J9712 plants were used as controls. e Sequence diagram of Bnamkk9 mutants generated via CRISPR/Cas9-mediated genome editing, with target sequences underlined and the protospacer-adjacent motif (PAM) in blue. f–i Evaluation of SSR resistance in Bnamkk9 mutants (T4 progeny) on leaves at 48 hpi (f, g) and on stems at 168 hpi (h, i). j, k Cell death at 72 h post-transfection (hpf) (j) and H2O2 accumulation measured with 3, 3’-diaminobenzidine (DAB) staining at 12 hpf (k) were observed in N. benthamiana leaves transiently expressing BnaA07.MKK9, BnaA07.MKK9KR, BnaA07.MKK9DD and EV, with red circles indicating infiltration zones. l Gene ontology enrichment analysis of differentially expressed genes between N. benthamiana leaves transiently expressing the BnaA07.MKK9DD and BnaA07.MKK9KR variants at 12 hpf. m, n Evaluation of SSR resistance in N. benthamiana transiently expressing BnaA07.MKK9KR and BnaA07.MKK9DD. S. sclerotiorum was inoculated in the infiltration areas at 12 hpf, and disease symptoms and lesion areas were photographed (m) and quantified (n) at 24 hpi. The upper part of the N. benthamiana leaf was the control without S. sclerotiorum inoculation. Scale bars: 1 cm (b, f, j, k, m) and 5 cm (d, h). In (a, c, g, i, n), bars represent the mean ± SD, and n represents the number of plants. Statistical significance was determined by a two-tailed Student’s t-test. In (a, c, g, i), significance compared with J9712. Source data are provided as a Source Data file.
