Fig. 1: Hematopoietic CD34+ cells from four independent del(5q) MDS patients were assayed by scRNAseq. | Nature Communications

Fig. 1: Hematopoietic CD34+ cells from four independent del(5q) MDS patients were assayed by scRNAseq.

From: Single-cell transcriptional profile of CD34+ hematopoietic progenitor cells from del(5q) myelodysplastic syndromes and impact of lenalidomide

Fig. 1

A CD34+ cells were obtained from bone marrow aspirates of newly diagnosed del(5q) MDS patients, healthy donors and patients treated with Lenalidomide, and were subjected to single-cell RNA sequencing and analysis. PCR partial cytogenetic responder, CCR complete cytogenetic responder, NR non-responder. Part of this figure was created with BioRender.com. B Uniform Manifold Approximation and Projection (UMAP) of 42,494 cells representing the expected 14 hematopoietic progenitors: HSC hematopoietic stem cells, LMPP lymphoid-primed multipotent progenitors, GMP granulocyte-monocyte progenitors; granulocyte progenitors; monocyte progenitors; dendritic cell progenitors; CLP common lymphoid progenitors; B-cell progenitors; T-cell progenitors; MEP megakaryocyte-erythroid progenitors; MK_Prog megakaryocyte progenitors; early erythroid progenitors; late erythroid progenitors; basophil progenitors. C Per patient UMAP showing the identity of the cells projected from the integrated space. D Dotplot showing the percentage and value of the normalized expression of the canonical marker genes used to assign the cell identity to each cluster. E Barplot representing the contribution of cells from each patient to the different cell types. F Barplot representing the number of cells assigned to each cell type for the studied patients. G Barplot representing the percentage of cells assigned to each cell type for del(5q) MDS patients and healthy samples. N = 7 biologically independent samples were used (n = 3 healthy donors and 4 del(5q) MDS patients). Data are presented as mean values +/−SD. Two-sided Wilcoxon signed-rank test was used to calculate statistical significance. Exact p-values for the differential abundance of each hematopoietic progenitor between the del(5q) MDS and the healthy condition were the following: HSC: p = 0.63; LMPP: p = 0.23; GMP: p = 0.06; Granulocyte: p = 0.23; Monocytes: p = 0.06; DendriticCell: p = 0.63; CLP: p = 1; pro-B: p = 0.23; T: p = 0.04; MEP: p = 0.11; MK_Prog: p = 0.63; EarlyErythroid: p = 1; LateErythroid: p = 0.23; Basophil: p = 0.4.

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