Fig. 6: Total IgG antibodies purified from rabbit sera following immunization with NP-cocktail protected humanized mice from lethal EBV challenge.

A Neutralization of Akata-EBV infection of HNE1 epithelial cells (top panel) and CNE2-EBV infection of Akata B cells (bottom panel) by IgG purified from sera of three rabbits immunized with the indicated vaccines. The half-maximal inhibitory concentration (IC₅₀) was calculated by sigmoid trend fitting. B Schematic illustration of humanized mice reconstitution and passive protection against EBV lethal challenge. i.v., intravenously; i.p., intraperitoneally. 53 female SCID-beige mice were used. The figure was created from Biorender.com. Titers of anti-gHgL (C), anti-gB (D) and anti-gp42 (E) rabbit IgG in sera from humanized mice. The black arrow indicates the administration of IgG and the yellow arrow indicates the administration of EBV. Data are shown as mean ± SEM (n = 8). F Body weight of mice post-EBV challenge. The yellow arrow indicates the day of EBV infection. Data are shown as mean ± SEM. Humanized mice in the mock group were not challenged with EBV. G Survival plot of humanized mice after EBV challenge. P values are shown in the Source data file. The color of the asterisks denotes the statistical difference. H, I EBV DNA copy numbers in peripheral blood of each group. Each line represents an individual mouse and the dashed line indicates the detection limit. H, I Summarize the same experiment and data have been separated in two graphs for clarity. J EBV DNA copy numbers in the spleen (left panel) and mesenteric lymph nodes (right panel) of humanized mice treated with different IgG. The dashed line indicates the detection limit. Samples of two mice in the PBS group which died on day 7 and 10 were not collected because of decay. Data are shown as mean ± SEM (n = 8 for the NP-gHgL, NP-gB, NP-gp42, NP-cocktail and Free-cocktail groups, n = 6 for the PBS group and n = 5 for the mock group). F, J Statistical analysis was performed using one-way ANOVA with Dunnett’s multiple comparison and precise P values are shown in the Source data file. The color of the asterisks or ns denotes the statistical difference. Source data are provided as a Source Data file.