Fig. 7: Humanized mice injected with IgG induced by NP-cocktail were free of EBV-associated lymphoma. | Nature Communications

Fig. 7: Humanized mice injected with IgG induced by NP-cocktail were free of EBV-associated lymphoma.

From: A cocktail nanovaccine targeting key entry glycoproteins elicits high neutralizing antibody levels against EBV infection

Fig. 7

A Representative macroscopic spleen and spleen sections stained by hematoxylin and eosin (HE), immunostained for hCD20+ and in situ hybridized for EBV-encoded RNA (EBER). Each image is representative of the experimental group (Scale bar = 50 μm) (NP-gHgL, NP-gB, NP-gp42, NP-cocktail and Free-cocktail: n = 8; PBS, n = 6; Mock, n = 5). B Spleen weight of individual mice in each group (NP-gHgL, NP-gB, NP-gp42, NP-cocktail and Free-cocktail: n = 8; PBS, n = 6; Mock, n = 5) at the end of the experiments or earlier at the time of euthanasia (>20% body weight loss). Samples of two mice of PBS group died on day 7 and 10 were not collected because of decay. Data are shown as mean ± SEM. Statistical analysis was performed using one-way ANOVA with Turkey’s multiple comparison test and the precise P values are shown in the source data file. The color of the asterisks or ns denotes the statistical difference. C 1/8 mice, 2/8 mice and 5/8 mice in NP-gHgL, Free-cocktail and PBS IgG-treated group, respectively, developed EBV-associated lymphoma, defined by histological observation. DNA copy numbers (D) and the percentages of hCD45+ (E), hCD3+ (F), hCD19+ (G) cells are shown for each tumor. The horizontal line represents the mean of each group and dashed line indicates the detection limit. (NP-gHgL, n = 1; Free-cocktail, n = 2; PBS, n = 5). H Representative macroscopic tumor and tumor tissue stained (NP-gHgL, n = 1; Free-cocktail, n = 2; PBS, n = 5) by HE, hybridized for EBER, and immunostained for hCD20+ at necropsy (Scale bar = 50 μm). Source data are provided as a Source Data file.

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