Fig. 3: NCF4 deficiency promotes tumorigenesis of CRC.
a Body weight change analysis of gender- and age-matched WT (n = 14), Ncf4+/– (n = 9), and Ncf4–/– (n = 13) mice after AOM injection at Day 0 and three rounds treatment of DSS (2%). b Survival analysis of WT (n = 24), Ncf4+/– (n = 14), and Ncf4–/– (n = 20) mice after AOM injection at Day 0 and three rounds of treatment of DSS (2%). c, d Colorectal tumors in WT (n = 13), Ncf4+/– (n = 7), and Ncf4–/– (n = 7) mice day 70 under AOM-DSS treatment. Scale bar: 10 mm. e H&E staining of Colorectal tumors in WT, Ncf4+/–, and Ncf4–/– mice in (c). Scale bars: 10 μm. f, g Immunoblot analysis of caspase-1 (f) and ELISA analysis of IL-18, IL-1β, TNF and IL-6 (g) in colon tissues from WT (n = 18), Ncf4+/– (n = 10), and Ncf4–/– mice (n = 8) in (c). h–j Colorectal tumors (h, i) and spleens (j) from gender- and age-matched offspring of ApcMin/+ mice crossed with Ncf4–/– mice with genotype as indicated (n = 8 for WT, n = 10 for Ncf4+/–, and n = 8 for Ncf4–/– in i). Scale bars: 10 mm for (h), and 5 mm for (j). Data are from 2 (b, g, i) or representative of 3 independent experiments with similar results (a, c, d–f, h, j). Data represent Mean ± SEM for (d, g, i), 2-sided Student’s t-test without multiple-comparisons correction, two-way ANOVA for (a), Log-rank (Mantel–Cox) test for (b), p-value is indicated in the graph. Source data are provided as a Source Data file.
