Fig. 5: Transmembrane domain engineering for proper expression and localization of protopine 6-hydroxylase (P6H). | Nature Communications

Fig. 5: Transmembrane domain engineering for proper expression and localization of protopine 6-hydroxylase (P6H).

From: Intein-mediated temperature control for complete biosynthesis of sanguinarine and its halogenated derivatives in yeast

Fig. 5

a Schematic of the chimeric P6H engineering strategy to address improper processing and localization in yeast. b Characterization of the activity of P6H variants from various species and engineered fusions in the PRO222 strain. c Investigation of the subcellular localization and expression of P6H variants by fusing EGFP at the C-terminus, visualized using fluorescence confocal microscopy. Micrographs were representative of at least two independent experiments. The significance of sanguinarine titer differences was calculated using two-way ANOVA followed by Sidak’s multiple comparisons test. Data are presented as mean ± s.d. (n  =  3 biologically independent samples). Source data are provided as a Source Data file.

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