Fig. 1: Blood electrolytes in Kir4.2+/+ and Kir4.2−/− animals following four days on 0 K diet.
From: Low potassium activation of proximal mTOR/AKT signaling is mediated by Kir4.2
Blood (A) K+(p = 0.034), (B) Na+ (p = 0.0059), (C) Cl− (p = 3 × 10−5), and (D) HCO3− (1.5 × 10−4) values in Kir4.2+/+ and Kir4.2−/− animals after four days on 0 K diet. Daily urine K+ (E) concentration and (F) excretion at baseline and on 0 K diet for indicated time. G Cumulative urine K+ losses on 0 K diet (p = 0.0068, 0.0002, <0.0001, <0.0001). H Urine K+ excretion normalized to creatinine from spot urines collected from animals at baseline and on 0 K diet for indicated times (p = 0.018, 0.0096). Daily urine Na+ (I) concentration and (J) excretion at baseline and on 0 K diet for indicated time. K Normalized daily hour urine Na+ excretion in mice consuming a 0 K diet for 1 day (p = 0.024). L Calculated free water clearance for mice consuming a 0 K diet for 1 day (p = 0.032). N = 5 per group for (A–D) and (L) except (A) where N = 5 for Kir4.2+/+ and 4 for Kir4.2−/−. One knockout blood K+ sample was excluded for the presence of gross hemolysis. N = 10 per group for (E–J) and 15 per group for (K). *P < 0.05 by unpaired t test. #P < 0.05 for genotype difference at indicated timepoints by two-way ANOVA with repeated measures followed by Sidak’s multiple comparison test. †P < 0.05 for interaction between treatment and time variables by two-way ANOVA with repeated measures. All tests were two-sided. Data presented as mean ± sem.
