Fig. 1: Cck2rb expressed in the pituitary FSH cells is essential for normal FSH expression.
From: Identification of the FSH-RH as the other gonadotropin-releasing hormone
a, b Double in situ hybridization of the pituitary of medaka labeled with fshb (a), lhb (b), and cck2rb (n = 4 fish). c–e Gross morphology of gonads (c) as well as ovarian size (d) and testis size (e) of cck2rb+/+ and cck2rb−/− females and males (n = 5 fish; ***, p = 2.3e-6, 1.9e-6). f Histological sections of the ovary and testis of cck2rb+/+ and cck2rb−/−. g The estradiol (E2) content of females was significantly lower than that of wild type medaka (n = 3 fish; p = 0.0038). h–m Expression of fshb (h, k), lhb (i, l), and tshb (j, m) in the pituitary of each genotype (n = 7 fish; ***, p = 3.2e-5, 9.2e-5, 9.8e-6). The data are mean ± SEM. ***p < 0.001, N.S. not significant, two-sided Dunnett’s test (d–e), two-sided Student’s t test (g–m). Source data are provided as a Source Data file.
