Fig. 3: Hypophysiotropic neurons expressing ccka and cckb exist in the hypothalamus.
From: Identification of the FSH-RH as the other gonadotropin-releasing hormone
a Luciferase assay of cholecystokinin family peptides CCK-8s and Gastrin-8s for cAMP, Ca2+, and MAPK pathways in HeLa cells expressing Cck2rb (n = 3 individual wells). The data are mean ± SEM. b In situ hybridization of ccka and cckb mRNA in the preoptic area (POA) and the nucleus ventralis tuberis (NVT), of the hypothalamus. c Double in situ hybridization of the ccka (magenta) and cckb (green) in the NVT (n = 4 fish). d, e Immunohistochemistry (IHC), using a CCK-specific antibody labeling the CCK neurons in the NVT (d) and axonal fibers in the pituitary (e) (n = 2 fish). f Dual labeling of retrogradely introduced biocytin (green) from the pituitary and ccka mRNA (magenta) (n = 3 fish). Source data are provided as a Source Data file.
