Fig. 5: TopI-dependent satellite transcription is conserved across eukaryotes.

a TopI inhibition decreases satellite transcription in mouse cells. RNAs from Mouse 3T3 cells treated with CPT (2.5 μM) were analyzed with real-time PCR. n = 4 biological replicates. b TopI knockdown decreases satellite transcription in mouse cells. RNAs from mouse 3T3 cells transfected with mTopI siRNA (#1 and #2) were analyzed with real-time PCR. n = 3 biological replicates. c TopI is required for DSB-induced satellite transcription in mouse cells. RNAs from mouse 3T3 cells treated with DMSO, CPT (2.5 μM), Etoposide (Etop, 30 μM), or Etoposide plus CPT (E+C) for 12 h, were analyzed with real-time PCR. n = 3 biological replicates. d TopI inhibition decreases satellite transcription in Drosophila cells. RNAs from Drosophila S2 cells treated with DMSO or CPT (10 μM) for 6 or 12 h were analyzed with real-time. n = 4 biological replicates. e TopI is required for DSB-induced satellite transcription in Drosophila cells. RNAs extracted from Drosophila S2 cells treated with DMSO, CPT (10 μM), Etoposide (Etop, 30 μM), triptolide (Trip, 4 μM) or Etoposide plus CPT (E+C) for 12 h. n = 3 biological replicates. f TopI promotes satellite transcription in Drosophila larvae tissues. RNAs from Drosophila larvae wing imaginal discs with mock or siTopI (#3 and #5) treatment were analyzed with real-time PCR. n = 3 biological replicates. g, h Satellite transcription is gradually elevated in a TopI-dependent manner in Drosophila tumor tissues. RNAs from control, notch-driven (g) or lethal (s) giant larvae (lgl) (h) Drosophila solid-tumor tissues with mock or siTopI treatment were analyzed with real-time PCR. n = 3 technical replicates. All Data here are presented as mean values +/− SEM. Two-sided Student’s T-test for (a, b, d, f, g, h) and ANOVA followed by pairwise comparisons using Tukey’s test for (c, e). ns, not significant (P > 0.1). Numeric values for P < 0.1 are shown. Source data are provided as Source Data file.