Fig. 5: ASIC3 drives CGRP release in psoriatic inflammation.
From: Sensory ASIC3 channel exacerbates psoriatic inflammation via a neurogenic pathway in female mice
a DRG neurons from Asic3+/+ and Asic3−/− mice were stimulated with acid (pH 5.5), acid plus amiloride (100 μM amiloride in pH 5.5) or GMQ (1 mM GMQ) for 30 min and CGRP was measured in the extracellular solution (ECS). n = 6 for ECS and pH 5.5 groups and n = 4 for pH 5.5 + AMI and GMQ groups. F(1,32) = 19.64, pH 5.5: ***p < 0.001; pH 5.5 + AMI: p = 0.9925; GMQ: ***p = 0.0006 < 0.001, Asic3−/− vs. Asic3+/+, two-way ANOVA. b DRG neurons from Asic3+/+ and Asic3−/− mice were stimulated with high potassium (30 mM KCl) for 30 min and CGRP was measured in neuronal supernatant. n = 4 for ECS and KCl groups. F(1,12) = 0.06706, KCl: p = 0.9462, Asic3−/− vs. Asic3+/+; F(1,12) = 44.40, Asic3+/+: **p = 0.0023 < 0.01, ECS vs. KCl, Asic3−/−: ***p = 0.0004 < 0.001, ECS vs. KCl; two-way ANOVA. c CGRP release ex vivo from skin punch biopsies of Asic3+/+ and Asic3−/− mice treated with IMQ. n = 8 mice per group. IMQ: F(1,28) = 5.869, *p = 0.0131 < 0.05, Asic3−/− vs. Asic3+/+, two-way ANOVA. d Representative images of skin tissues isolated from Asic3+/+ and Asic3−/– mice treated with vehicle or IMQ and stained with anti-CGRP (green), anti-PGP9.5 (red) and DAPI (blue). Scale bar, 100 μm. e Relative CGRP intensity normalized by DAPI in (d). IMQ: F(1,16) = 9.945, ***p = 0.0008 < 0.001, Asic3−/− vs. Asic3+/+, two-way ANOVA. n = 5 for each group. Summary data are mean ± SEM.
