Fig. 6: ASIC3 drives psoriatic inflammation through CGRP release.
From: Sensory ASIC3 channel exacerbates psoriatic inflammation via a neurogenic pathway in female mice
a Schematic protocol of CGRP receptor antagonism in Asic3+/+ mice and local CGRP supplement in Asic3−/− mice. b Spleen weight of mice in (a). Asic3+/+: ***p = 0.0008 < 0.001, vehicle vs. BIBN4096, two-tailed unpaired Student’s t test. Asic3−/−: *p = 0.0269 < 0.05, vehicle vs. CGRP, two-tailed unpaired Student’s t test. c Representative H&E staining (left) and quantification of epidermal thickness (middle and right) of lesional skin after CGRP receptor antagonism in Asic3+/+ and local CGRP supplement in Asic3−/− mice. Scale bar, 200 μm. Asic3+/+: ***p = 5.6092E−5 < 0.001, vehicle vs. BIBN4096, two-tailed unpaired Student’s t test. Asic3−/−: ***p = 2.5174E−6 < 0.001, vehicle vs. CGRP, two-tailed unpaired Student’s t test. d Representative Ki67 staining (left) and quantification of Ki67+ cells (middle and right) in lesional skin after CGRP receptor antagonism in Asic3+/+ and local CGRP supplement in Asic3−/− mice. Scale bar, 200 μm. Asic3+/+: **p = 0.0043 < 0.01, vehicle vs. BIBN4096, two-tailed unpaired Student’s t test. Asic3−/−: **p = 0.0049 < 0.01, vehicle vs. CGRP, two-tailed unpaired Student’s t test. e Psoriasis-related cytokine IL-17, IL-22, and IL-23 protein expression in lesional skin after CGRP receptor antagonism in Asic3+/+ and local CGRP supplement in Asic3−/− mice. Asic3+/+: IL-17: **p = 0.0060 < 0.01; IL-22: **p = 0.0011 < 0.01; IL-23: *p = 0.0167 < 0.05, vehicle vs. BIBN4096, two-tailed unpaired Student’s t test. Asic3−/−: IL-17: **p = 0.0051 < 0.01; IL-22: **p = 0.0043 < 0.01; IL-23: *p = 0.0232 < 0.05, vehicle vs. CGRP, two-tailed unpaired Student’s t test. n = 8 mice per group. Summary data are mean ± SEM.
