Fig. 6: DSB inhibition does not restore oogenesis in mios^-/-.

a Sex ratio graph for 60 dpf+ mios; spo11^uc73 double heterozygous, heterozygous mutant, mutant wildtype, mutant heterozygous, and double mutant fish. Pink represents female fish and blue indicates male fish. Number of fish screened are indicated for each group. Statistical significance was evaluated by performing a two-tailed χ² test with Bonferroni correction against the mios^+/-; spo11^+/uv73 control group. P = 0.0125. Source data are provided as a Source Data file. b Germ cell (Ddx4, teal) and DNA (DAPI, white) in mios^-/- (mios;spo11 mutant wildtype/mutant heterozygous fish, n = 6) and (c) mios^-/-; spo11^uc73/uc73 double mutant fish (n = 7). Developing oocytes (b) and sperm (c) are outlined with dashed lines, respectively. Scale bar is 50 µM.