Fig. 8: Effects of 22 on WalK activity. | Nature Communications

Fig. 8: Effects of 22 on WalK activity.

From: Asymmetric total synthesis of polycyclic xanthenes and discovery of a WalK activator active against MRSA

Fig. 8

a Transcription levels of genes regulated by WalKR in the presence or absence of 22 (0.1 μg/mL). b Lysostaphin-induced lysis process in the presence or absence of 22 (0.025 μg/mL). c Biofilm formation of different strains in the presence or absence of 22 (0.03, 0.06 μg/mL). Three biologically independent experiments at least were performed in a–c. The mean is shown, and error bars represent the s.d. P values were determined using a nonparametric one-way ANOVA. d SPR analysis demonstrated the binding of 22 to WalK. e 22 docked with the erWalK structure (PDB ID: 5IS1). The space-filling model shows the binding of 22 in the β-sheet pocket. f 22 docked with the erWalKR86C structure (PDB ID: 7DUD). The protein residues, hydrogen bonds and hydrophobic bonds are shown in a solid-ribbon and in 2D view. Pi-Donor hydrogen bonds, conventional hydrogen bonds and hydrophobic bonds are colored orange, green and purple, respectively. Source data are provided as a Source Data file.

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