Fig. 3: Mucorales infection activates the HIF1α pathway in human airway epithelial cells.

A Immunoblot representing HIF1α accumulation from whole cell lysates collected 1 or 3 h post-infection of HSAEC1-KT cells with C. bertholletiae. B Densitometric analysis of the immunoblot in (A). Data represents the mean ± SEM of three independent experiments performed in singlicate. Two-tailed Student’s t test was used for statistical analysis. C Schematic of transwell experiment. D Immunoblot representing HIF1α accumulation from whole cell lysates collected 3 h post-infection of HSAEC1-KT cells with R. delemar under standard conditions or in a transwell. E Densitometric analysis of the immunoblot in panel D. Data represents the mean ± SEM of three independent experiments performed in singlicate. Two-tailed Student t test was used for statistical analysis. ns, not significant. F HSAEC1-KT cells were infected with R. delemar for 3 h and localization of HIF1α was assessed by indirect immunofluorescence with an anti-HIF1α antibody (green). Hoechst stain was used to visualize nuclei (blue). DIC, differential inference contrast. Scale bars = 100 μm. Microscopy experiments were performed three times independently with similar results.