Fig. 3: Phenotypic diversity of AMPO-formation in microbacteria.
From: The lactonase BxdA mediates metabolic specialisation of maize root bacteria to benzoxazinoids
Phylogenetic tree constructed from whole genome alignment of 39 microbacteria. Tips are coloured by the host plant from which the strains were isolated. The column “plate assay” shows the AMPO classification (strong AMPO-former or non-AMPO-former) of the strains based on red colour formation on 100% TSA plates supplemented with MBOA (200 µg/mL). The adjacent columns display the classifications of metabolite analyses (MBOA, AMPO, HMPAA and DIMBOA-Glc) of liquid 50% TSB cultures supplemented with 500 µM MBOA (“MBOA metabolization”) or DIMBOA-Glc (“DIMBOA-Glc metabolization”) after 68 h. The column “MBOA C-source” refers to the assay where the strains were grown in minimal medium supplemented with 500 µM MBOA as a sole carbon source (based on mean results of 12 independent replicates grown in two independent runs). Columns “OG000xxxx” report copy numbers of gene orthogroups (Supplementary Data 2) that are unique and specific to AMPO-formers.
