Fig. 1: Identification of GMGTs synthase by in vivo and in vitro assays. | Nature Communications

Fig. 1: Identification of GMGTs synthase by in vivo and in vitro assays.

From: Biosynthesis of GMGT lipids by a radical SAM enzyme associated with anaerobic archaea and oxygen-deficient environments

Fig. 1

a GMGT-0 biosynthesis pathway. b UPLC-MS extracted ion chromatograms of lipid extracts from M. maripaludis with plasmid pMEV4-tes showing production of GDGT-0, and with plasmid pMEV4-gms-tes showing production of GMGT-0. The in vitro biochemical reaction reveals the synthesis of GMGT-0 via incubation of the purified Gms protein with GDGT-0, SAM and DTH at 37 °C for 72 h.

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