Fig. 1: VZV gC binds type II IFN.
From: Viral modulation of type II interferon increases T cell adhesion and virus spread
a Schematic representation of VZV gC (top) and recombinant soluble gC constructs that were used in this study (below). All constructs contain a BiP signal peptide at the N-terminus and a Twin-Strep-tag. The first and last gC residue is indicated in each construct. The numbering of amino acid residues corresponds to the sequence of the Dumas strain. AA amino acid, SP signal peptide, ECD extracellular domain, TM transmembrane domain, CD cytoplasmic domain, R2 repeated domain 2, BiP Drosophila immunoglobulin binding chaperone protein signal sequence, Strep Twin-Strep-tag with enterokinase site for optional removal of tag. b Sensorgram showing the results of a binding screening between VZV gC and cytokines using the Biacore X100 system. The recombinant purified VZV gCP23-V531 was immobilised on a CM5 chip (3600 RU). IFNs and TNFα were injected at 100 nM with a flow rate of 10 μL/min. One representative experiment out of three biological repetitions is shown. s seconds, RU resonance units.
