Fig. 6: Chemotherapy induces JAK1-dependent STAT6-Y641 phosphorylation and nuclear translocation.
From: Co-targeting JAK1/STAT6/GAS6/TAM signaling improves chemotherapy efficacy in Ewing sarcoma
a, b IB analysis of cytoplasmic and nuclear fractions from MHH-ES-1 cells treated with SN-38 (a) or etoposide (b) for 24 h. Cyto cytoplasm, Nuc nucleus. For quantification, STAT6 localization in cytoplasm or nuclei is determined by relative fluorescent intensity and at least 100 cells were used in each quantification. c Representative IF images from biological triplicates using MHH-ES-1 cells stably expressing EV or HA-STAT6 treated with or without SN-38 (1 μM) for 24 h. The scale bar represents 20 µm. The scale bar represents 20 µm. d, e IB analysis of WCL from MHH-ES-1 cells treated with indicated doses of SN-38 (d) or 1 μM SN-38 for indicated time periods (e). f–h IB analysis of WCL from MHH-ES-1 cells treated with indicated compounds for 24 h. IL-4, 1 ng/mL; AS1517499, 5 μM; SN-38, 1 μM; etoposide, 10 μM. i–k IB analysis of WCL from MHH-ES-1 cells treated with indicated compounds for 24 h. IL-4, 1 ng/mL; filgotinib, 5 μM; SN-38, 1 μM; etoposide, 10 μM. WB data presented in this figure are representative data from experimental duplicates.
