Fig. 4: Polyadenylation of mRNAs encoding components of deadenylase complex is impaired in mouse and human oocytes matured in vitro.

a, b Normalized counts of Btg4 (left) and the four Cnot family (right) genes (Cnot6, Cnot6l, Cnot7, Cnot8) in the in vitro and in vivo MII oocytes for two biological replicates of mice (a) or combined data of humans (b). Individual data points are shown on the plots. Fold changes for each gene are shown on top of the column. c, d Box plot for the poly(A) tail length of Btg4 and the four Cnot family genes in GV and MII oocytes matured in vivo or in vitro in mice (c) and humans (d). The n numbers for the box plots are 12900, 17360, 8618, 112, 63, 37, 1666, 2072, 1574, 1986, 871, 820, 933, 141, and 95 for (c) and 4326, 1164, 2887, 358, 343, 727, 412, 155, 386, 264, 197, 389, 184, 76, and 57 for (d) from left to right. For all the box plots, the “×” indicates the mean value, the central line represents the median value, the top and bottom of the box represent the value of the 25th and 75th percentile, and the boundary of the lower and the upper whiskers represent the minimum and the maximum value of the data set, respectively. The p values are derived from a two-tailed Student’s t-test. Source data are provided as a Source Data file.