Fig. 1: Cyprocide-B is a selective nematicide with broad-spectrum activity in PPNs.

a POR knockdown screen results. Difference in survival percentage between POR knockdown and wild-type conditions is plotted for the 87 unique nematicides tested. b Structure of selectivin-A and the three POR-dependent selective nematicides. The shared DODA core scaffold is highlighted in purple. c Summarized screening results for the DODA nematicide library in C. elegans larval (Cel L1) and dauer stages, M. hapla embryo (Mh Egg) and infective juvenile (Mh J2) stages, D. dipsaci (Dd), and P. penetrans (Pp). Relative viability is represented by the color-coded scale. Library compounds are organized by structural similarity. d The Cyprocides are shown clustered by structural similarity with screening results from (c) overlaid. Family members (nodes) are connected by an edge if they share a Tanimoto structural similarity score >0.725. Named analogs are labeled on the nodes. The enrichment of Cyprocides within screen hits and associated one-sided hypergeometric p-values are indicated. e Dose-response analysis of cyprocide-B and tioxazafen on nematode and non-target organisms, including human cells (HEK293 and HepG2), fungi (Saccharomyces cerevisiae and Candida albicans), plant beneficial rhizobacteria (Pseudomonas simiae and P. defensor), zebrafish (D. rerio), and Drosophila melanogaster (D. mel) adult and larval stages. As a positive control for the D. melanogaster assays we found 50 μM abamectin killed 100% of adult flies (n = 3 biological replicates (BRs)). Compound activity is represented by the color-coded scale. Black indicates the condition was not tested. Source data are provided as a Source data file.