Fig. 4: Cyprocide-B is bioactivated into a toxic electrophile by P450s in PPNs.

EICs for indicated cyprocide-B parent and metabolite m/z values from D. dipsaci buffer after exposure to a DMSO, b cyprocide-B, and c 1-ABT plus cyprocide-B. d EIC peak area at indicated retention time (RT) for cyprocide-B parent and metabolites in D. dipsaci buffer after incubation with cyprocide-B+/− 1-ABT (n = 4 BRs). Exact p-values are as follows: Parent (p = 0.000642), GSH (p = 0.000014), γGluCys (p = 0.000757), Sulfoxide (p = 0.000236), CysGly (p = 0.002462), Cys (p = 0.022239). e D. dipsaci were preincubated +/− NACET before a three-day incubation in cyprocide-B or fluopyram. The proportion of mobile worms after chemical exposure is reported (n = 4 BRs for cyprocide-B exposures, n = 3 BRs for fluopyram exposures). f Mean AUC of M. incognita P450-expressing yeast strains exposed to 50 μM cyprocide-B, normalized to the solvent control for each strain (n = 6 BRs). g Cyprocide-B dose-response for yeast strains expressing CYP4731A3 and EV controls. Mean AUC relative to solvent controls is indicated by the color-coded scale (n = 4 BRs). h, i EICs for indicated cyprocide-B parent and metabolite m/z values from yeast lysates after cyprocide-B exposure in EV and CYP4731A3-expressing strains. j Number of M. incognita eggs per milligram of tomato root tissue after 6 weeks of infection in soil drenched with solvent or indicated chemical at 60 μM is reported (n = 6 BRs). k Tomato root weight at infection assay endpoint in each condition (n = 6 BRs). In all panels error bars represent SEM, p-values were obtained from unpaired two-tailed Student’s t-tests, ns is not significant (p > 0.05). Source data are provided as a Source data file.