Fig. 3: Acute stimulus-evoked effects of psilocin on PVN reactivity and behavior in females.

Fiber photometry: Psilocin n = 8, Vehicle n = 8; Behavior: Psilocin n = 12, Vehicle n = 12; A Baseline PVN reactivity (left): ΔF/F trace plots of changes following 500 ms air-puff. Comparisons were made using a 2-way ANOVA. Data points represent 500 ms binned group averages with mean +/- S.E.M. (shaded area); Heatmaps (top right) comparing individual responses to air-puff (dotted line) in vehicle and psilocin groups. Average AUC and PP + /- S.E.M. (bottom right) compared by 2-tailed unpaired t-tests between groups. Each data point represents an individual subject. B Average maximum speed (left) and distance traveled (right) after air-puff stimulus. A 2-way ANOVA was performed for statistical analysis. Data points are group averages + /- S.E.M. (shaded area) (C) Day of injection PVN reactivity (left): ΔF/F trace plots of changes following exposure to 500 ms air-puff. A 2-way ANOVA was performed for statistical analysis. Data points represent group averages within 500 ms binned window +/- S.E.M. (shaded area); Heatmaps (top right) comparing individual responses to air-puff (dotted line) in vehicle and psilocin groups.; Average AUC and PP + /- S.E.M (bottom right) compared by 2-tailed unpaired t-tests between groups. Each data point represents an individual subject. D Average maximum speed (left) and distance traveled (right) after air-puff stimulus. Comparisons were made using a 2-way ANOVA. Data points are group averages +/- S.E.M (shaded area). In each trace bin plot, a significant increase in ΔF/F was determined whenever the lower bound of the 99% CI was >0 with statistical significance shown as colored lines above each ΔF/F curve with colors corresponding to the respective binned traces. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. AUC = area under curve, PP = peak point, ΔF/F = change in fluorescence as a function of baseline fluorescence, CI = confidence interval.