Fig. 6: Effects of psilocin on PVN reactivity following an acute stress in male and females. | Nature Communications

Fig. 6: Effects of psilocin on PVN reactivity following an acute stress in male and females.

From: Increased reactivity of the paraventricular nucleus of the hypothalamus and decreased threat responding in male rats following psilocin administration

Fig. 6

A Average male (Psilocin n = 9; Vehicle n = 9) and female (Psilocin n = 11, Vehicle n = 9) plasma corticosterone levels. 2-way ANOVA revealed a main effect of sex (p < 0.0001). Each data point represents an individual subject. Mean +/- S.E.M. compared between groups. B Male post-restraint PVN reactivity (left): ΔF/F trace plots of changes following exposure to a 500 ms air-puff A 2-way ANOVA was performed for statistical analysis. Data points represent group averages within 500 ms binned window + /- S.E.M. (shaded area); Heatmaps (top right) comparing individual responses to air-puff stimulus (dotted line) in vehicle and psilocin groups. Average AUC and PP + /- S.E.M. (bottom right) compared by unpaired 2-tailed t-test between groups. Each data point represents an individual subject (Psilocin n = 7, Vehicle n = 6). C Male: Average maximum speed (left) and distance traveled (right) following air-puff stimulus. 2-way ANOVA was performed for statistical analysis. Data points are group averages + /- S.E.M. (shaded area) (Psilocin n = 9, Vehicle n = 9) (D) Female post-restraint PVN reactivity (left): ΔF/F trace plots of changes following exposure to a 500 ms air-puff. 2-way ANOVA was performed for statistical analysis. Data points represent group averages within 500 ms binned window +/- S.E.M. (shaded area); Heatmaps (top right) comparing individual responses to air-puff stimulus (dotted line) in vehicle and psilocin groups. Average AUC and PP + /- S.E.M. (bottom right) compared by unpaired 2-tailed t-test between groups. Each data point represents an individual subject. (Psilocin n = 7, Vehicle n = 5). E Female: Average maximum speed (left) and distance traveled (right) after air-puff stimulus. 2-way ANOVA was performed for statistical analysis. Data points are group averages +/- S.E.M. (shaded area) (Psilocin n = 11, Vehicle n = 9). In each trace bin plot, a significant increase in ΔF/F was determined whenever the lower bound of the 99% CI was >0 with statistical significance shown as colored lines above each ΔF/F curve with colors corresponding to the respective binned traces *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. AUC = area under curve, PP = peak point, ΔF/F = change in fluorescence as a function of baseline fluorescence, CI = confidence interval.

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