Fig. 2: Effect of cellular levels of RodA on MreB dynamics.
From: Molecular motor tug-of-war regulates elongasome cell wall synthesis dynamics in Bacillus subtilis
Exemplar kymographs of MreB filament dynamics at low (a) and high (b) RodA levels achieved through expression from an IPTG-inducible promoter. Kymographs are measured around the cell circumference. More examples are shown in Supplementary Fig. 11. c Time MreB filaments spend in each motion state as a function of rodA expression level. Single-molecule switching rates for MreB processive subtracks (d) and static subtracks (e). Solid coloured lines represent medians of all data points for each condition. Error bars represent 95% CI. f, g MreB filament speed and processivity for processive subtracks. White filled circles: median of biological replicates. Horizontal dashed lines: value of each parameter (eg rate, speed) at native rodA expression level in strain SM01 (mreB-HaloTag, Δhag). Narrow horizontal lines: median of all data points. Violin plots: thick error bar lines indicate IQR, thin error bar lines indicate 1.5× IQR. Strain used: B. subtilis SM28 (mreB-HaloTag, Pspac-rodA, Δhag). Further quantification in Supplementary Fig. 12. Sample sizes and numbers of experimental replicates are listed in Supplementary Table 7. Effect sizes are listed in Supplementary Table 2.
