Fig. 4: Effect of cellular levels of RodA on cell shape and MreB filament density.
From: Molecular motor tug-of-war regulates elongasome cell wall synthesis dynamics in Bacillus subtilis
Effect of titration of RodA cellular levels expressed as sole cellular copy from an inducible promotor on (a) cell diameter, and (b) surface density of motile MreB filaments measured by SIM-TIRF microscopy. Horizontal lines show overall median. Violin plots: thick error bar lines indicate IQR, thin error bar lines indicate 1.5× IQR. White filled circles indicate biological replicates. Grey filled circles indicate medians of each field of view in SIM-TIRF experiments. Red line indicates values for native RodA levels. Strain used: B. subtilis SM28 (mreB-HaloTag, Pspac-rodA, Δhag). Sample sizes and numbers of experimental replicates are listed in Supplementary Table 7. Effect sizes are listed in Supplementary Table 2.
