Fig. 1: Human thyroid cancer single-cell cell landscape reveals low immune infiltration in MTC. | Nature Communications

Fig. 1: Human thyroid cancer single-cell cell landscape reveals low immune infiltration in MTC.

From: The neurotransmitter calcitonin gene-related peptide shapes an immunosuppressive microenvironment in medullary thyroid cancer

Fig. 1

A Workflow of our study. The left panel showed the experimental design of single-cell RNA-sequencing (scRNA-seq) as the discovery cohort. The right panel showed the type of validation experiments, including bulk-RNA sequencing, immunohistochemistry (IHC), and multi-immunohistochemical staining (mIHC), and their corresponding cohorts. Created with BioRender.com, released under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International license. B Uniform manifold approximation and projection (UMAP) visualization of 228,400 cells from tumors and adjacent normal tissues, colored by cell type annotations. ILCs innate lymphoid cells, NK natural killer cell, DC dendritic cell, PVL perivascular cell, imPVL immature perivascular cell, dPVL differentiated perivascular cell. C Heatmap showing the expression patterns of marker genes in major cell types. D Cell type proportions of immune cells and non-immune cells. Each stacked bar represents one cancer type. PTC Papillary thyroid cancer, MTC Medullary thyroid cancer, ATC Anaplastic thyroid cancer, BC Breast cancer, GC Gastric cancer, HCC Hepatocellular carcinoma, ICC Intrahepatic cholangiocarcinoma, PRAD Prostate cancer, PDAC Pancreatic cancer, GBM Glioblastoma. E Heatmap showing the distribution ratio of major cell types in PTC and MTC. Calculated by Chi-square test, *p < 0.01. F Bar graph showing the predicted immune score of PTC (28 samples from our cohort and 502 samples from TCGA public dataset) and 8 samples MTC in the bulk-RNA data. The box plot illustrates the interquartile range in relation to the median, while the middle lines represent the median, and the lower and upper hinges denote the 25–75% interquartile range (IQR), with whiskers extending up to a maximum of 1.5 times IQR. P-value was determined using two-sided Wilcoxon rank-sum test. G IHC staining of CD45 in the tumor region of PTC and MTC (left panel). Dot plot shows the proportion of CD45+ cells in PTC and MTC (right panel). P-values from two-tailed Student’s t test were represented. Source data are provided as a Source Data file.

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