Fig. 2: ELA rearing caused sex-specific changes in threat-induced Ca + activity of CeAL CRF + neurons. | Nature Communications

Fig. 2: ELA rearing caused sex-specific changes in threat-induced Ca + activity of CeAL CRF + neurons.

From: Central amygdala CRF+ neurons promote heightened threat reactivity following early life adversity in mice

Fig. 2

a Experimental design. b Representative image of viral expression. c Activity traces during CS + onset. d CRF + neuron activity was elevated during CS + onset (3-way-RM-ANOVA tone F(1,26) = 68.76, p = 8.9e-9, η2 = 1.60; 3-way-RM-ANOVA sex F(1,26) = 1.14, p = 0.70, η2 = 0.07; 3-way-RM-ANOVA rearing F(1,26) = 0.17, p = 0.20, η2 = 0.24; 3-way-RM-ANOVA sex x rearing F(1,26) = 0.01, p = 0.23, η2 = 0.02). n = 8 (F Ctrl, F ELA), 7 (M Ctrl, M ELA). e Peak activity was not impacted by rearing or sex (2-way-ANOVA sex F(1,26) = 0.05, p = 0.81, η2 = 0.04; 2-way-ANOVA rearing F(1,26) = 0.59, p = 0.44, η2 = 0.11; 2-way-ANOVA sex x rearing F(1,26) = 0.36, p = 0.55, η2 = 0.10). n = 8 (F Ctrl, F ELA), 7 (M Ctrl, M ELA). f Latency to peak z-score was not impacted by rearing or sex (2-way-ANOVA sex F(1,26) = 0.004, p = 0.94, η2 = 0.01; 2-way-ANOVA rearing F(1,26) = 0.78, p = 0.38, η2 = 0.14; 2-way-ANOVA sex x rearing F(1,26) = 1.01, p = 0.32, η2 = 0.19. n = 8 (F Ctrl, F ELA), 7 (M Ctrl, M ELA). g Activity traces during CS + (t = 0-30) and WN (t = 30) aligned to t = 0. h Activity during the CS+ was increased in ELA females (p = 0.0006) and suppressed in ELA males (p = 0.002) compared to Ctrls (3-way-RM-ANOVA rearing x sex F(1,26) = 6.06, p = 0.02, η2 = 0.62, Bonferroni’s multiple comparisons). n = 8 (F Ctrl, F ELA), 7 (M Ctrl, M ELA). i Activity traces during noise alone (NA; t = 120) aligned to t = 0. j Activity was enhanced in ELA females (p = 0.02) and suppressed in ELA males (p = 0.04; 3-way-RM-ANOVA rearing x sex F(1, 26) = 5.70, p = 0.02, η2 = 0.78, Bonferroni’s multiple comparisons). n = 8 (F Ctrl, F ELA), 7 (M Ctrl, M ELA). k Activity to the WN was decreased by ELA (3-way-RM-ANOVA rearing F(1, 26) = 5.91, p = 0.02, η2 = 0.75, cue F(1, 26) = 0.003, p = 0.95, η2 = 0.10). n = 8 (F Ctrl, F ELA), 7 (M Ctrl, M ELA). l Startle was negatively correlated with AUC following WN (Two-tailed Spearman R = −0.354, p = 0.05). m AUC prior to WN was negatively correlated with AUC following WN (Two-tailed Spearman R = −0.483, p = 0.006). n AUC to CS + onset was positively correlated with AUC following WN (Two-tailed Spearman R = 0.416, p = 0.02). o AUC during CS + was positively correlated with AUC prior to WN (Two-tailed Spearman R = 0.821, p = 2.8e-8). Plots depict individual mice and means ± SEM. *< 0.05, **< 0.01, ***< 0.001, ****< 0.0001. Source data are provided as a Source Data file. Figure 2a was created with BioRender.com and released under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International license.

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