Fig. 1: Chlamydomonas reinhardtii transcriptional response to osmotic stress. | Nature Communications

Fig. 1: Chlamydomonas reinhardtii transcriptional response to osmotic stress.

From: Multi-omics analysis of green lineage osmotic stress pathways unveils crucial roles of different cellular compartments

Fig. 1

a Gene ontology term analysis of the transcriptional response of Chlamydomonas cells treated with 100 mM NaCl or 300 mM mannitol at the indicated time points (‘and h indicate minutes and hours respectively). See also Supplementary Figs. 1–3 and Supplementary Data 1, 2. b Confocal images of fluorescently tagged Chlamydomonas cells with different fluorescent markers under control conditions and treatment with 100 mM NaCl for 30 h. Cellular markers used: Endosomes, Cre16.g661700: REMORIN, labels the Golgi apparatus and secretory pathway (REM:VENUS); Starch, Cre17.g721500: STARCH SYNTHASE 2 (STA2), labels pyrenoid starch and starch granules (STA2:VENUS); flagella, Cre12.g485050: CARBONIC ANHYDRASE 6 (CAH6) labels flagella (CAH6:VENUS). (Right) Quantification of the number of vesicles in REMORIN cells, STA2 puncta and flagella length in control and cells treated with NaCl. n = 20 cells per condition. Cell images were taken in two independent experiments. Scale bars = 10 μm. Center lines show medians and box limits indicate the 25th and 75th percentiles. Whiskers represent minima and maxima, P values are from a one-way ANOVA test between control condition and treatments, p < 0.05. See also Source Data.

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