Fig. 3: Identification of genes with roles in osmotic stress in Chlamydomonas. | Nature Communications

Fig. 3: Identification of genes with roles in osmotic stress in Chlamydomonas.

From: Multi-omics analysis of green lineage osmotic stress pathways unveils crucial roles of different cellular compartments

Fig. 3

a Upper panel, diagrammatic representation of barcode mutant screens. Unique barcodes allow genome-wide screening of a Chlamydomonas mutant pool. Mutants sensitive to osmotic stress can be identified because their barcodes will be less abundant after growth under treatment (NaCl, Mannitol, PEG, hypoosmotic) compared to control conditions. Lower panel, diagrammatic representation of high confidence hits (FDR < 0.3). Cre indicates Chlamydomanas specific genes. Hit orthology to S. cerevisiae (Sc) or A. thaliana (At) based on reciprocal BLAST (<1E-10). * and ** represents an osmotic phenotype previously described in Sc or At homologous genes. See also Supplementary Data 5, 6 and Source Data. b Heat map representing the significance of high confidence hits (FDR < 0.3). The x-axis indicates the different conditions screened, NaCl, Mannitol, PEG, and hypoosmotic stress grouped in the different replicas performed. Y-axis contains the gene ID and annotation. See also Supplementary Data 6 and Source Data. c Confocal images of Chlamydomonas cells showing the subcellular localization of proteins fused to VENUS fluorescent protein. Cartoon represents different organelles in Chlamydomonas: Chl chloroplast, GA Golgi apparatus, C cytoplasm, CV contractile vacuole. Red color shows chloroplast autofluorescence and yellow shows VENUS fluorescence. White arrows indicate the base of the flagella. Scale bar; 1 μm.

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