Fig. 4: Conserved osmotic stress pathways across the green lineage show different spatiotemporal cell death patterns in Arabidopsis roots.

a Representative growth vessels of 4 days post inoculation Chlamydomonas cells growing in control media (CTL), 100 mM NaCl (NaCl) and 300 mM Mannitol (Mannitol). (right) Growth quantification of mutant strains upon stress were normalized to growth of wild-type upon stress. Duplicate cell counts were performed in three independent replicates.* represents p < 0.05 (two-way ANOVA). Scale bar = 500 mm. b Arabidopsis primary roots 4 days post-transfer to 140 mM NaCl or 300 mM mannitol, black lines indicate transfer side, scale bar = 1 mm. (right) Quantification of root growth after transfer to 140 mM NaCl or 300 mM mannitol, root growth ratio upon transfer was normalized to the growth of wild-type upon stress treatment. * Indicates significance, two-way ANOVA test interactions between genotype and treatment, p < 0.05. See also Supplementary Fig. 7 and Supplementary Data 9 and Source Data for raw data and statistical information. c Confocal images of 4 days post germination root tips, 15 h after transfer to 140 mM NaCl. * Indicates cell death. Scale bar = 100 μm. See Supplementary Fig. 9 for controls. d Confocal images of 4 days post germination root tips, 15 h after transfer to 300 mM mannitol. * Indicates cell death. Scale bar = 100 μm. See Supplementary Fig. 9 for controls. e Quantification of root cell death after transfer to 140 mM NaCl. n = 10 roots for each genotype in three independent replicates. f Quantification of root cell death after transfer to 300 mM mannitol. n = 10 roots for each genotype in three independent replicates.