Fig. 6: O-GlyNAcylation of MITF contributes to palbociclib resistance by regulating senescence signaling.
From: O-GlcNAcylation of MITF regulates its activity and CDK4/6 inhibitor resistance in breast cancer
a The pie chart shows the distribution of MITF-associated sites across the genomic features in MCF-7 PR cells. b The clustering and distribution of MITF-associated sites on genomic DNA around the transcriptional starting sites in MCF-7 PR and MCF-7 cells. c ChIP-seq to reveal the association of MITF at the promoter regions of the SERPINE1, IL-6, CSF1, and PTGER2 genes using IGV software. d ChIP-qPCR was used to validate the results shown in c, TYR was used as a positive control (n = 3 independent experiments). e, f MCF-7 PR cells (e) and T-47D PR cells (f) were collected after treatments as indicated and subjected to qPCR to examine the expression of genes as indicated (n = 3 independent experiments). g–h MCF-7 PR cells (g) and T-47D PR cells (h) were collected after treatments as indicated and subjected to qPCR to examine the expression of genes as indicated (n = 3 independent experiments). i MCF-7 PR and T-47D PR cells were collected after treatments as indicated, followed by immunoblotting for the indicated proteins. (n = 3 independent experiments). j SA-β-gal staining of MCF-7 PR cells treated as indicated (n = 3 independent experiments). The scale bar represents 20 μm. Right, quantification of results shown on left. **p ≤ 0.01, *p ≤ 0.05. All error bars are expressed as mean ± SEM. Two-tailed Student’s t tests were employed for statistical evaluation. Source data are provided as a Source Data file.
