Fig. 1: SARS-CoV-2 RNA and neutralizing antibody prevalence in wildlife communities.
From: Widespread exposure to SARS-CoV-2 in wildlife communities
a Represents counties where swabs were collected (both wildlife rehabilitation centers and study sites). Gray scale indicates sample sizes. Circles represent SARS-CoV-2 positive samples and color indicates species in (b). Prevalence of (b) RT-qPCR (quantitative reverse transcription polymerase chain reaction) detections collected between May 2022 and September 2023 (n = 757). Detections include species with >10 individuals sampled, and black points indicate prevalence (percentage of individuals with positive detections) and 95% confidence intervals (See Supplementary Table 1 for full dataset that includes all 23 species, n = 789). c Shows seroprevalence measured using plaque reduction neutralization tests (PRNT) for species sampled both prior to SARS-CoV-2 arrival in 2020 (n = 49) and samples collected in our study between June and July 2022 (n = 67, Supplementary Data 2). The size of each point in (c) is in relation to the percent neutralization. Samples with a 60% or greater percent neutralization were considered positive samples. Solid color points represent seroprevalence estimates (percentage of individuals positive for neutralizing antibodies) and error bars represent 95% confidence intervals. d Represents percent neutralization values of serum samples to SARS-CoV-2 sampled both prior (n = 49) and post (n = 67) SARS-CoV-2 arrival. The black dotted horizontal line represents a 60% neutralization cut-off. The purple diamond represents neutralizing antibody titers for a Virginia opossum who was found conclusively RT-qPCR positive one month prior. Box plots show the median (center line) and interquartile range (25th–75th percentile of the data) and whiskers indicate range of data 1.5 times the interquartile below and above the 25th and 75th percentile, respectively. Gray boxes represent samples with less than a 60% neutralization value, and black boxes represent samples with greater than a 60% neutralization value. All data points represent samples taken from individual animals which serve as biological replicates. For each PRNT we performed three technical replicates, which were averaged for each individual. Organism silhouettes were sourced from PhyloPic82.
