Fig. 2: KAP1 localizes to the gene bodies and 3′ ends of IEGs upon serum stimulation.

a HA (KAP1) ChIP-Seq metagene analysis at 16-fold IEGs in the three indicated conditions. See legend for sample identification. b HA (KAP1) ChIP-Seq browser track of FOS in the four indicated conditions. See legend for sample identification. c, d HA ChIP-Seq quantitation of KAP1 density at 4-fold IEGs (n = 69) and 16-fold IEGs (n = 16) at c PP regions and d GB regions. Data represents the Log₂FC value for the respective sample (see X-axis) normalized to serum 0 min in DMSO-treated cells using ChIP-Seq signal from 2 biological replicates. The n number represents the number of genes in each cluster plotted. The Tukey plots indicate the median (black center line), the first and third quartiles (edges of the box), and the 1.5× interquartile range below and above the box as whiskers. Dots are presented as genes with normalized signals beyond these defined ranges. Statistics were calculated between the dTAG plus serum treatment condition and the respective condition shown on the Tukey plot. Two-sided Wilcoxon signed-rank test. P-values are indicated. e HA (KAP1) ChIP-Seq metagene analysis of all genome-wide KAP1 peaks (n = 12,665) in the three indicated conditions. See legend for sample identification. f HA (KAP1) ChIP-Seq browser track at the SFPQ locus in the four indicated conditions. See legend for sample identification. Source data are provided as a Source Data file.