Fig. 7: DNAgel promoted wound healing and completed skin regeneration.

Six full-thickness skin defects, 1 cm in diameter, were created symmetrically along the spine on the back of each rat using a hole punch and were treated according to the group. a Representative wound images during healing process on Day 0, 3, 5, 7, 10. Scale bar: 2 mm. b Wound traces at different days. c Wound size and d healing rate at different days for each group, according to Extent of healing (%) = \(\left(1-\,\frac{{A}_{t}}{{A}_{0}}\right)\times 100\%\). Data were presented as mean ± SD (n = 3). e Representative images of immunohistochemistry staining with CK14 (red) and DAPI (blue) on Day 7 (Wound closure time points in DNAgel group) and Day 10 (Total skin regeneration time point in DNAgel group) for each group. Scale bar: 100 μm. f Hematoxylin and Eosin staining, Masson’s Trichrome staining and Sirius Red staining on Day 7 and 10 of the newly regenerated skin tissues for each group. The purple dotted line marks the initial trauma margin. Scale bar: 1 mm (top); Scale bar: 100 μm (bottom, enlarged). Statistical analysis was performed by one-way ANOVA with Tukey’s multiple comparisons (c, d). Representative images are shown from two (a, e, f) independent experiments with similar results. Source data are provided as a Source Data file.