Fig. 2: SHP2 epigenetic activity is dependent on AR for its nuclear translocation. | Nature Communications

Fig. 2: SHP2 epigenetic activity is dependent on AR for its nuclear translocation.

From: SHP2 as a primordial epigenetic enzyme expunges histone H3 pTyr-54 to amend androgen receptor homeostasis

Fig. 2

a VCaP cells were treated with 1 μM of (R)-9b, SHP099, Enz or Abr for 8 h and lysates were IP with SHP2 antibodies, followed by immunoblotting with ACK1 antibody (top panel). Lysates were also IP with pY54-H3 antibodies, followed by immunoblotting with H3 antibody (2nd panel). Lower panels are immunoblots with the indicated antibodies. b VCaP cells were treated as above and nuclear and cytosolic extracts were prepared. The lysates were immunoblotted with the indicated antibodies. c HEK293T cells were transfected with FLAG-tagged SHP2 or mutants expressing constructs with ACK1 and AR for 48 h and nuclear and cytosolic fractionation was performed. Nuclear lysates were IP with FLAG antibodies, followed by immunoblotting with pTyr antibody (top panel). Lower panels are immunoblots with the indicated antibodies. d VCaP, and LAPC-4 cells were transfected with ACK1/TNK2, AR and PTPN11 siRNAs and lysates were IP with pY54-H3 antibody, followed by immunoblotting with H3 antibody (top panel). Lower panels are immunoblots with the indicated antibodies. For a–d, representative images are shown from n = 3 biologically independent experiments. Source data are provided as a Source Data file.

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